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venus tagged mus muluscus b arrestin2  (Addgene inc)


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    Addgene inc venus tagged mus muluscus b arrestin2
    Venus Tagged Mus Muluscus B Arrestin2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/venus+tagged+mus+muluscus+b+arrestin2/mVenus-N1+(Plasmid+%2354640)/pm32470395-680-250-258
    Average 93 stars, based on 17 article reviews
    venus tagged mus muluscus b arrestin2 - by Bioz Stars, 2026-10
    93/100 stars

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    Generated:

    Article Title: β-Arrestin-Biased Allosteric Modulator of NTSR1 Selectively Attenuates Addictive Behaviors.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Gq/11-deficient (DGNAQ and GNA11) HEK293 - Clone 1 Alvarez-Curto et al., 2016 N/A Parental HEK293 line fromwhich b-arrestin and Gq null lines were generated Alvarez-Curto et al., 2016 N/A G protein-deficient (DGNAS, GNAL, GNAQ, GNA11, GNA12, and GNA13) HEK293 - Clone 38 Inoue et al., 2012 N/A U2OS cells stably expressing b-arrestin2-GFP and 3xHA-NTSR1 Barak et al., 2016 N/A HEK293 cells stably expressing mitochondriallytargeted apoaequorin Barak et al., 2016 N/A Experimental Models: Organisms/Strains Mouse: C57BL/6J: WT The Jackson Laboratory Cat# 000664 Mouse: DAT KO: Slc6a3tm1 Giros et al., 1996 N/A Mouse: b-arrestin2 KO: Arrb2tm1Rjl Bohn et al., 1999; The Jackson Laboratory Cat# 011130 Mouse:b-arrestin2f/f: Arrb2tm1(flox)nu Urs et al., 2016 N/A Mouse:D1R-Cre/b-arrestin2f/f:Drd1tm1(Cre)/ Arrb2tm1(flox)nu Urs et al., 2016 N/A Mouse:D2R-Cre/b-arrestin2f/f:Drd2tm1(Cre)/ Arrb2tm1(flox)nu Urs et al., 2016 N/A Mouse:A2aR-Cre/b-arrestin2f/f: Adora2tm1(Cre)/ Arrb2tm1(flox)nu Urs et al., 2016 N/A Mouse: DAT-Cre/b-arrestin2f/f: Slc6a3tm1(cre)/ Arrb2tm1(flox)nu Toth et al., 2018 N/A Recombinant DNA NTSR1: N-terminal 3xHA-tagged human neurotensin receptor 1 (NTSR1) (wild-type) cloned into pcDNA3.1+ (Invitrogen) at KpnI (50) and XbaI (30) cDNA Resource Center Cat# NTSR10TN00 NTSR1-GFP: N-terminal 3xHA-tagged human neurotensin receptor 1 (NTSR1) (wild-type) cloned into eGFPN3 (Clontech) Barak et al., 2016 N/A NTSR1-FAP: N-terminal MarsCy1-tagged human neurotensin receptor 1 (NTSR1) (wild-type) in pcDNA3.1+ (Invitrogen) Barak et al., 2016 N/A Mitochondrial-targeted apoaequorin expression vector Rizzuto et al., 1992 N/A Inositol-1,4,5-trisphosphate BRET sensor Gulyás et al., 2015 N/A Gaq chimeric protein plasmid Inoue et al., 2012 N/A NTSR1-Rluc: C-terminal Rluc-tagged humanNTSR1 in the pRluc-N vector (BioSignal Packard Cat. #6310220) Caron Laboratory N/A b-arrestin2-Venus: C-terminal Venus-tagged mus muluscus b-arrestin2 in the N1-mVenus vector (Addgene plasmid #54640) Pack et al., 2018 N/A GRK2-FLG: C-terminal Flag-tagged Bos taurus GRK2 in pcDNA1/Amp vector Pitcher et al., 1998 N/A AP-TGFa plasmid Inoue et al., 2012 N/A Software and Algorithms Image Studio Lite version 5.2 LI-COR https://www.licor.com/bio/ image-studio-lite/ Prism, version 7 GraphPad https://www.graphpad.com/ Statmate, version 2 GraphPad https://www.graphpad.com/ (Continued on next page) ll Cell 181, 1364–1379.e1–e10, June 11, 2020 e2 Article

    Stable Transfection:

    Article Title: β-Arrestin-Biased Allosteric Modulator of NTSR1 Selectively Attenuates Addictive Behaviors.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Gq/11-deficient (DGNAQ and GNA11) HEK293 - Clone 1 Alvarez-Curto et al., 2016 N/A Parental HEK293 line fromwhich b-arrestin and Gq null lines were generated Alvarez-Curto et al., 2016 N/A G protein-deficient (DGNAS, GNAL, GNAQ, GNA11, GNA12, and GNA13) HEK293 - Clone 38 Inoue et al., 2012 N/A U2OS cells stably expressing b-arrestin2-GFP and 3xHA-NTSR1 Barak et al., 2016 N/A HEK293 cells stably expressing mitochondriallytargeted apoaequorin Barak et al., 2016 N/A Experimental Models: Organisms/Strains Mouse: C57BL/6J: WT The Jackson Laboratory Cat# 000664 Mouse: DAT KO: Slc6a3tm1 Giros et al., 1996 N/A Mouse: b-arrestin2 KO: Arrb2tm1Rjl Bohn et al., 1999; The Jackson Laboratory Cat# 011130 Mouse:b-arrestin2f/f: Arrb2tm1(flox)nu Urs et al., 2016 N/A Mouse:D1R-Cre/b-arrestin2f/f:Drd1tm1(Cre)/ Arrb2tm1(flox)nu Urs et al., 2016 N/A Mouse:D2R-Cre/b-arrestin2f/f:Drd2tm1(Cre)/ Arrb2tm1(flox)nu Urs et al., 2016 N/A Mouse:A2aR-Cre/b-arrestin2f/f: Adora2tm1(Cre)/ Arrb2tm1(flox)nu Urs et al., 2016 N/A Mouse: DAT-Cre/b-arrestin2f/f: Slc6a3tm1(cre)/ Arrb2tm1(flox)nu Toth et al., 2018 N/A Recombinant DNA NTSR1: N-terminal 3xHA-tagged human neurotensin receptor 1 (NTSR1) (wild-type) cloned into pcDNA3.1+ (Invitrogen) at KpnI (50) and XbaI (30) cDNA Resource Center Cat# NTSR10TN00 NTSR1-GFP: N-terminal 3xHA-tagged human neurotensin receptor 1 (NTSR1) (wild-type) cloned into eGFPN3 (Clontech) Barak et al., 2016 N/A NTSR1-FAP: N-terminal MarsCy1-tagged human neurotensin receptor 1 (NTSR1) (wild-type) in pcDNA3.1+ (Invitrogen) Barak et al., 2016 N/A Mitochondrial-targeted apoaequorin expression vector Rizzuto et al., 1992 N/A Inositol-1,4,5-trisphosphate BRET sensor Gulyás et al., 2015 N/A Gaq chimeric protein plasmid Inoue et al., 2012 N/A NTSR1-Rluc: C-terminal Rluc-tagged humanNTSR1 in the pRluc-N vector (BioSignal Packard Cat. #6310220) Caron Laboratory N/A b-arrestin2-Venus: C-terminal Venus-tagged mus muluscus b-arrestin2 in the N1-mVenus vector (Addgene plasmid #54640) Pack et al., 2018 N/A GRK2-FLG: C-terminal Flag-tagged Bos taurus GRK2 in pcDNA1/Amp vector Pitcher et al., 1998 N/A AP-TGFa plasmid Inoue et al., 2012 N/A Software and Algorithms Image Studio Lite version 5.2 LI-COR https://www.licor.com/bio/ image-studio-lite/ Prism, version 7 GraphPad https://www.graphpad.com/ Statmate, version 2 GraphPad https://www.graphpad.com/ (Continued on next page) ll Cell 181, 1364–1379.e1–e10, June 11, 2020 e2 Article

    Expressing:

    Article Title: β-Arrestin-Biased Allosteric Modulator of NTSR1 Selectively Attenuates Addictive Behaviors.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Gq/11-deficient (DGNAQ and GNA11) HEK293 - Clone 1 Alvarez-Curto et al., 2016 N/A Parental HEK293 line fromwhich b-arrestin and Gq null lines were generated Alvarez-Curto et al., 2016 N/A G protein-deficient (DGNAS, GNAL, GNAQ, GNA11, GNA12, and GNA13) HEK293 - Clone 38 Inoue et al., 2012 N/A U2OS cells stably expressing b-arrestin2-GFP and 3xHA-NTSR1 Barak et al., 2016 N/A HEK293 cells stably expressing mitochondriallytargeted apoaequorin Barak et al., 2016 N/A Experimental Models: Organisms/Strains Mouse: C57BL/6J: WT The Jackson Laboratory Cat# 000664 Mouse: DAT KO: Slc6a3tm1 Giros et al., 1996 N/A Mouse: b-arrestin2 KO: Arrb2tm1Rjl Bohn et al., 1999; The Jackson Laboratory Cat# 011130 Mouse:b-arrestin2f/f: Arrb2tm1(flox)nu Urs et al., 2016 N/A Mouse:D1R-Cre/b-arrestin2f/f:Drd1tm1(Cre)/ Arrb2tm1(flox)nu Urs et al., 2016 N/A Mouse:D2R-Cre/b-arrestin2f/f:Drd2tm1(Cre)/ Arrb2tm1(flox)nu Urs et al., 2016 N/A Mouse:A2aR-Cre/b-arrestin2f/f: Adora2tm1(Cre)/ Arrb2tm1(flox)nu Urs et al., 2016 N/A Mouse: DAT-Cre/b-arrestin2f/f: Slc6a3tm1(cre)/ Arrb2tm1(flox)nu Toth et al., 2018 N/A Recombinant DNA NTSR1: N-terminal 3xHA-tagged human neurotensin receptor 1 (NTSR1) (wild-type) cloned into pcDNA3.1+ (Invitrogen) at KpnI (50) and XbaI (30) cDNA Resource Center Cat# NTSR10TN00 NTSR1-GFP: N-terminal 3xHA-tagged human neurotensin receptor 1 (NTSR1) (wild-type) cloned into eGFPN3 (Clontech) Barak et al., 2016 N/A NTSR1-FAP: N-terminal MarsCy1-tagged human neurotensin receptor 1 (NTSR1) (wild-type) in pcDNA3.1+ (Invitrogen) Barak et al., 2016 N/A Mitochondrial-targeted apoaequorin expression vector Rizzuto et al., 1992 N/A Inositol-1,4,5-trisphosphate BRET sensor Gulyás et al., 2015 N/A Gaq chimeric protein plasmid Inoue et al., 2012 N/A NTSR1-Rluc: C-terminal Rluc-tagged humanNTSR1 in the pRluc-N vector (BioSignal Packard Cat. #6310220) Caron Laboratory N/A b-arrestin2-Venus: C-terminal Venus-tagged mus muluscus b-arrestin2 in the N1-mVenus vector (Addgene plasmid #54640) Pack et al., 2018 N/A GRK2-FLG: C-terminal Flag-tagged Bos taurus GRK2 in pcDNA1/Amp vector Pitcher et al., 1998 N/A AP-TGFa plasmid Inoue et al., 2012 N/A Software and Algorithms Image Studio Lite version 5.2 LI-COR https://www.licor.com/bio/ image-studio-lite/ Prism, version 7 GraphPad https://www.graphpad.com/ Statmate, version 2 GraphPad https://www.graphpad.com/ (Continued on next page) ll Cell 181, 1364–1379.e1–e10, June 11, 2020 e2 Article

    Recombinant:

    Article Title: β-Arrestin-Biased Allosteric Modulator of NTSR1 Selectively Attenuates Addictive Behaviors.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Gq/11-deficient (DGNAQ and GNA11) HEK293 - Clone 1 Alvarez-Curto et al., 2016 N/A Parental HEK293 line fromwhich b-arrestin and Gq null lines were generated Alvarez-Curto et al., 2016 N/A G protein-deficient (DGNAS, GNAL, GNAQ, GNA11, GNA12, and GNA13) HEK293 - Clone 38 Inoue et al., 2012 N/A U2OS cells stably expressing b-arrestin2-GFP and 3xHA-NTSR1 Barak et al., 2016 N/A HEK293 cells stably expressing mitochondriallytargeted apoaequorin Barak et al., 2016 N/A Experimental Models: Organisms/Strains Mouse: C57BL/6J: WT The Jackson Laboratory Cat# 000664 Mouse: DAT KO: Slc6a3tm1 Giros et al., 1996 N/A Mouse: b-arrestin2 KO: Arrb2tm1Rjl Bohn et al., 1999; The Jackson Laboratory Cat# 011130 Mouse:b-arrestin2f/f: Arrb2tm1(flox)nu Urs et al., 2016 N/A Mouse:D1R-Cre/b-arrestin2f/f:Drd1tm1(Cre)/ Arrb2tm1(flox)nu Urs et al., 2016 N/A Mouse:D2R-Cre/b-arrestin2f/f:Drd2tm1(Cre)/ Arrb2tm1(flox)nu Urs et al., 2016 N/A Mouse:A2aR-Cre/b-arrestin2f/f: Adora2tm1(Cre)/ Arrb2tm1(flox)nu Urs et al., 2016 N/A Mouse: DAT-Cre/b-arrestin2f/f: Slc6a3tm1(cre)/ Arrb2tm1(flox)nu Toth et al., 2018 N/A Recombinant DNA NTSR1: N-terminal 3xHA-tagged human neurotensin receptor 1 (NTSR1) (wild-type) cloned into pcDNA3.1+ (Invitrogen) at KpnI (50) and XbaI (30) cDNA Resource Center Cat# NTSR10TN00 NTSR1-GFP: N-terminal 3xHA-tagged human neurotensin receptor 1 (NTSR1) (wild-type) cloned into eGFPN3 (Clontech) Barak et al., 2016 N/A NTSR1-FAP: N-terminal MarsCy1-tagged human neurotensin receptor 1 (NTSR1) (wild-type) in pcDNA3.1+ (Invitrogen) Barak et al., 2016 N/A Mitochondrial-targeted apoaequorin expression vector Rizzuto et al., 1992 N/A Inositol-1,4,5-trisphosphate BRET sensor Gulyás et al., 2015 N/A Gaq chimeric protein plasmid Inoue et al., 2012 N/A NTSR1-Rluc: C-terminal Rluc-tagged humanNTSR1 in the pRluc-N vector (BioSignal Packard Cat. #6310220) Caron Laboratory N/A b-arrestin2-Venus: C-terminal Venus-tagged mus muluscus b-arrestin2 in the N1-mVenus vector (Addgene plasmid #54640) Pack et al., 2018 N/A GRK2-FLG: C-terminal Flag-tagged Bos taurus GRK2 in pcDNA1/Amp vector Pitcher et al., 1998 N/A AP-TGFa plasmid Inoue et al., 2012 N/A Software and Algorithms Image Studio Lite version 5.2 LI-COR https://www.licor.com/bio/ image-studio-lite/ Prism, version 7 GraphPad https://www.graphpad.com/ Statmate, version 2 GraphPad https://www.graphpad.com/ (Continued on next page) ll Cell 181, 1364–1379.e1–e10, June 11, 2020 e2 Article

    Plasmid Preparation:

    Article Title: β-Arrestin-Biased Allosteric Modulator of NTSR1 Selectively Attenuates Addictive Behaviors.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Gq/11-deficient (DGNAQ and GNA11) HEK293 - Clone 1 Alvarez-Curto et al., 2016 N/A Parental HEK293 line fromwhich b-arrestin and Gq null lines were generated Alvarez-Curto et al., 2016 N/A G protein-deficient (DGNAS, GNAL, GNAQ, GNA11, GNA12, and GNA13) HEK293 - Clone 38 Inoue et al., 2012 N/A U2OS cells stably expressing b-arrestin2-GFP and 3xHA-NTSR1 Barak et al., 2016 N/A HEK293 cells stably expressing mitochondriallytargeted apoaequorin Barak et al., 2016 N/A Experimental Models: Organisms/Strains Mouse: C57BL/6J: WT The Jackson Laboratory Cat# 000664 Mouse: DAT KO: Slc6a3tm1 Giros et al., 1996 N/A Mouse: b-arrestin2 KO: Arrb2tm1Rjl Bohn et al., 1999; The Jackson Laboratory Cat# 011130 Mouse:b-arrestin2f/f: Arrb2tm1(flox)nu Urs et al., 2016 N/A Mouse:D1R-Cre/b-arrestin2f/f:Drd1tm1(Cre)/ Arrb2tm1(flox)nu Urs et al., 2016 N/A Mouse:D2R-Cre/b-arrestin2f/f:Drd2tm1(Cre)/ Arrb2tm1(flox)nu Urs et al., 2016 N/A Mouse:A2aR-Cre/b-arrestin2f/f: Adora2tm1(Cre)/ Arrb2tm1(flox)nu Urs et al., 2016 N/A Mouse: DAT-Cre/b-arrestin2f/f: Slc6a3tm1(cre)/ Arrb2tm1(flox)nu Toth et al., 2018 N/A Recombinant DNA NTSR1: N-terminal 3xHA-tagged human neurotensin receptor 1 (NTSR1) (wild-type) cloned into pcDNA3.1+ (Invitrogen) at KpnI (50) and XbaI (30) cDNA Resource Center Cat# NTSR10TN00 NTSR1-GFP: N-terminal 3xHA-tagged human neurotensin receptor 1 (NTSR1) (wild-type) cloned into eGFPN3 (Clontech) Barak et al., 2016 N/A NTSR1-FAP: N-terminal MarsCy1-tagged human neurotensin receptor 1 (NTSR1) (wild-type) in pcDNA3.1+ (Invitrogen) Barak et al., 2016 N/A Mitochondrial-targeted apoaequorin expression vector Rizzuto et al., 1992 N/A Inositol-1,4,5-trisphosphate BRET sensor Gulyás et al., 2015 N/A Gaq chimeric protein plasmid Inoue et al., 2012 N/A NTSR1-Rluc: C-terminal Rluc-tagged humanNTSR1 in the pRluc-N vector (BioSignal Packard Cat. #6310220) Caron Laboratory N/A b-arrestin2-Venus: C-terminal Venus-tagged mus muluscus b-arrestin2 in the N1-mVenus vector (Addgene plasmid #54640) Pack et al., 2018 N/A GRK2-FLG: C-terminal Flag-tagged Bos taurus GRK2 in pcDNA1/Amp vector Pitcher et al., 1998 N/A AP-TGFa plasmid Inoue et al., 2012 N/A Software and Algorithms Image Studio Lite version 5.2 LI-COR https://www.licor.com/bio/ image-studio-lite/ Prism, version 7 GraphPad https://www.graphpad.com/ Statmate, version 2 GraphPad https://www.graphpad.com/ (Continued on next page) ll Cell 181, 1364–1379.e1–e10, June 11, 2020 e2 Article

    Bioluminescence Resonance Energy Transfer:

    Article Title: β-Arrestin-Biased Allosteric Modulator of NTSR1 Selectively Attenuates Addictive Behaviors.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Gq/11-deficient (DGNAQ and GNA11) HEK293 - Clone 1 Alvarez-Curto et al., 2016 N/A Parental HEK293 line fromwhich b-arrestin and Gq null lines were generated Alvarez-Curto et al., 2016 N/A G protein-deficient (DGNAS, GNAL, GNAQ, GNA11, GNA12, and GNA13) HEK293 - Clone 38 Inoue et al., 2012 N/A U2OS cells stably expressing b-arrestin2-GFP and 3xHA-NTSR1 Barak et al., 2016 N/A HEK293 cells stably expressing mitochondriallytargeted apoaequorin Barak et al., 2016 N/A Experimental Models: Organisms/Strains Mouse: C57BL/6J: WT The Jackson Laboratory Cat# 000664 Mouse: DAT KO: Slc6a3tm1 Giros et al., 1996 N/A Mouse: b-arrestin2 KO: Arrb2tm1Rjl Bohn et al., 1999; The Jackson Laboratory Cat# 011130 Mouse:b-arrestin2f/f: Arrb2tm1(flox)nu Urs et al., 2016 N/A Mouse:D1R-Cre/b-arrestin2f/f:Drd1tm1(Cre)/ Arrb2tm1(flox)nu Urs et al., 2016 N/A Mouse:D2R-Cre/b-arrestin2f/f:Drd2tm1(Cre)/ Arrb2tm1(flox)nu Urs et al., 2016 N/A Mouse:A2aR-Cre/b-arrestin2f/f: Adora2tm1(Cre)/ Arrb2tm1(flox)nu Urs et al., 2016 N/A Mouse: DAT-Cre/b-arrestin2f/f: Slc6a3tm1(cre)/ Arrb2tm1(flox)nu Toth et al., 2018 N/A Recombinant DNA NTSR1: N-terminal 3xHA-tagged human neurotensin receptor 1 (NTSR1) (wild-type) cloned into pcDNA3.1+ (Invitrogen) at KpnI (50) and XbaI (30) cDNA Resource Center Cat# NTSR10TN00 NTSR1-GFP: N-terminal 3xHA-tagged human neurotensin receptor 1 (NTSR1) (wild-type) cloned into eGFPN3 (Clontech) Barak et al., 2016 N/A NTSR1-FAP: N-terminal MarsCy1-tagged human neurotensin receptor 1 (NTSR1) (wild-type) in pcDNA3.1+ (Invitrogen) Barak et al., 2016 N/A Mitochondrial-targeted apoaequorin expression vector Rizzuto et al., 1992 N/A Inositol-1,4,5-trisphosphate BRET sensor Gulyás et al., 2015 N/A Gaq chimeric protein plasmid Inoue et al., 2012 N/A NTSR1-Rluc: C-terminal Rluc-tagged humanNTSR1 in the pRluc-N vector (BioSignal Packard Cat. #6310220) Caron Laboratory N/A b-arrestin2-Venus: C-terminal Venus-tagged mus muluscus b-arrestin2 in the N1-mVenus vector (Addgene plasmid #54640) Pack et al., 2018 N/A GRK2-FLG: C-terminal Flag-tagged Bos taurus GRK2 in pcDNA1/Amp vector Pitcher et al., 1998 N/A AP-TGFa plasmid Inoue et al., 2012 N/A Software and Algorithms Image Studio Lite version 5.2 LI-COR https://www.licor.com/bio/ image-studio-lite/ Prism, version 7 GraphPad https://www.graphpad.com/ Statmate, version 2 GraphPad https://www.graphpad.com/ (Continued on next page) ll Cell 181, 1364–1379.e1–e10, June 11, 2020 e2 Article

    Software:

    Article Title: β-Arrestin-Biased Allosteric Modulator of NTSR1 Selectively Attenuates Addictive Behaviors.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Gq/11-deficient (DGNAQ and GNA11) HEK293 - Clone 1 Alvarez-Curto et al., 2016 N/A Parental HEK293 line fromwhich b-arrestin and Gq null lines were generated Alvarez-Curto et al., 2016 N/A G protein-deficient (DGNAS, GNAL, GNAQ, GNA11, GNA12, and GNA13) HEK293 - Clone 38 Inoue et al., 2012 N/A U2OS cells stably expressing b-arrestin2-GFP and 3xHA-NTSR1 Barak et al., 2016 N/A HEK293 cells stably expressing mitochondriallytargeted apoaequorin Barak et al., 2016 N/A Experimental Models: Organisms/Strains Mouse: C57BL/6J: WT The Jackson Laboratory Cat# 000664 Mouse: DAT KO: Slc6a3tm1 Giros et al., 1996 N/A Mouse: b-arrestin2 KO: Arrb2tm1Rjl Bohn et al., 1999; The Jackson Laboratory Cat# 011130 Mouse:b-arrestin2f/f: Arrb2tm1(flox)nu Urs et al., 2016 N/A Mouse:D1R-Cre/b-arrestin2f/f:Drd1tm1(Cre)/ Arrb2tm1(flox)nu Urs et al., 2016 N/A Mouse:D2R-Cre/b-arrestin2f/f:Drd2tm1(Cre)/ Arrb2tm1(flox)nu Urs et al., 2016 N/A Mouse:A2aR-Cre/b-arrestin2f/f: Adora2tm1(Cre)/ Arrb2tm1(flox)nu Urs et al., 2016 N/A Mouse: DAT-Cre/b-arrestin2f/f: Slc6a3tm1(cre)/ Arrb2tm1(flox)nu Toth et al., 2018 N/A Recombinant DNA NTSR1: N-terminal 3xHA-tagged human neurotensin receptor 1 (NTSR1) (wild-type) cloned into pcDNA3.1+ (Invitrogen) at KpnI (50) and XbaI (30) cDNA Resource Center Cat# NTSR10TN00 NTSR1-GFP: N-terminal 3xHA-tagged human neurotensin receptor 1 (NTSR1) (wild-type) cloned into eGFPN3 (Clontech) Barak et al., 2016 N/A NTSR1-FAP: N-terminal MarsCy1-tagged human neurotensin receptor 1 (NTSR1) (wild-type) in pcDNA3.1+ (Invitrogen) Barak et al., 2016 N/A Mitochondrial-targeted apoaequorin expression vector Rizzuto et al., 1992 N/A Inositol-1,4,5-trisphosphate BRET sensor Gulyás et al., 2015 N/A Gaq chimeric protein plasmid Inoue et al., 2012 N/A NTSR1-Rluc: C-terminal Rluc-tagged humanNTSR1 in the pRluc-N vector (BioSignal Packard Cat. #6310220) Caron Laboratory N/A b-arrestin2-Venus: C-terminal Venus-tagged mus muluscus b-arrestin2 in the N1-mVenus vector (Addgene plasmid #54640) Pack et al., 2018 N/A GRK2-FLG: C-terminal Flag-tagged Bos taurus GRK2 in pcDNA1/Amp vector Pitcher et al., 1998 N/A AP-TGFa plasmid Inoue et al., 2012 N/A Software and Algorithms Image Studio Lite version 5.2 LI-COR https://www.licor.com/bio/ image-studio-lite/ Prism, version 7 GraphPad https://www.graphpad.com/ Statmate, version 2 GraphPad https://www.graphpad.com/ (Continued on next page) ll Cell 181, 1364–1379.e1–e10, June 11, 2020 e2 Article



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    Addgene inc venus tagged mus muluscus b arrestin2
    Venus Tagged Mus Muluscus B Arrestin2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/venus+tagged+mus+muluscus+b+arrestin2/mVenus-N1+(Plasmid+%2354640)/pm32470395-680-250-258
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